This resource is a collection of lesson plans for activities that promote student engagement with the concept of gene regulation, particularly in prokaryotes. A total of 4 active learning activities are described in detail, including "Lactose Operon Role Play," "Cell Growth in the Presence of Two Sugar Sources," "Design a Repressor System," and Alterations of Operon Function through Mutation." Activity caveats, assessment suggestions, and supplemental resources are all included.